Occurrence of Human Cytomegalovirus Glycoprotein B Genotypes in Immunocompetent and Immunosuppressed Taiwanese Patients

Objectives: To investigate the differences of genotypic distributions among isolates between immunosuppressed and immunocompetent patients in a Taiwanese population. Methods: Human cytomegalovirus (HCMV) isolates from 76 patients with adequate chart data were analyzed. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to define gB genotypes which include gB1 to gB4. The clinical data of the 76 patients were retrospectively collected by chart review and classified into an immunosuppressed (n = 32) or immunocompetent (n = 44) group. Results: Among the 32 immunosuppressed patients, the most commonly identified HCMV genotypes were gB1 (27/32, 84.3%) and gB3 (4/32, 12.5%). 59.1% (26/44) of the immunocompetent patients were infected by gB1 while 38.6% (17/44) of them were infected by gB3. The frequency of gB1 infection in the immunosuppressed group was significantly higher than that in the immunocompetent group (p = 0.025). However, there was no statistically significant difReceived: October 28, 2009 Accepted after revision: October 25, 2010 Published online: January 14, 2011 Yu-Jiun Chan, MD, PhD Division of Virology, Department of Pathology and Laboratory Medicine Taipei Veterans General Hospital, No. 201, Section 2 Shih-Pai Road, Taipei 11247, Taiwan (ROC) Tel. +886 2 2875 7297, Fax +886 2 2831 1311, E-Mail yjchan @ vghtpe.gov.tw © 2011 S. Karger AG, Basel 0300–5526/11/0544–0196$38.00/0 Accessible online at: www.karger.com/int K.-G.W. and M.-C.H. contributed equally to this article. This research was presented in part at the 11th Western Pacific Congress of Chemotherapy and Infectious Diseases (WPCCID), Taipei, Taiwan, ROC, December 2, 2008, PS3-012. D ow nl oa de d by : 54 .1 91 .4 0. 80 9 /1 6/ 20 17 8 :2 7: 07 P M